论文部分内容阅读
观察草苁蓉环烯醚萜苷(IGBR)对二乙基亚硝胺(DEN)诱发肝癌大鼠肝组织病理变化,探讨IGBR的抗氧化作用。Wistar大鼠分为对照组、模型组、5-FU组及IGBR组。除对照组,各组大鼠第一天腹腔注射200mg/kg DEN1次,然后给予饮用0.05%的DEN水溶液;5-FU组大鼠腹腔注射5-FU25mg/kg每周3次;IGBR组大鼠灌胃500mg/kgIGBR每日1次。实验第28周末处死大鼠,观察肝组织病理变化,用比色法检测肝组织抗氧化酶活性和丙二醛含量。与对照组相比,模型组肝细胞核大且深染,肝细胞变性、异型增生,某些增生灶可见癌变细胞。与模型组相比,5-FU组与IGBR组肝癌大肝组织SOD、CAT及GSH-Px活性升高,MDA水平降低,差异均有统计学意义(P均<0.05),但两组间无明显差异(P>0.05)。IGBR可通过提高肝组织抗氧化活性,对DEN诱发肝癌大鼠肝组织具有保护作用。
To observe the pathological changes of hepatic tissue of HCC rat induced by diethylnitrosamine (DEN) and investigate the anti-oxidative effect of IGBR. Wistar rats were divided into control group, model group, 5-FU group and IGBR group. In addition to the control group, rats in each group were intraperitoneally injected with 200mg / kg DEN 1 times on the first day, and then drank 0.05% DEN aqueous solution. 5-FU 25mg / kg was intraperitoneally injected in the 5-FU group three times a week; Gavage 500mg / kgIGBR once daily. At the end of the 28th week, the rats were sacrificed and pathological changes of liver were observed. The activities of antioxidant enzymes and malondialdehyde in liver tissue were detected by colorimetry. Compared with the control group, the model group of large and deep liver cell nuclei, hepatocellular degeneration, dysplasia, some hyperplasia visible cancerous cells. Compared with the model group, the activities of SOD, CAT and GSH-Px in the liver of 5-FU and IGBR groups were increased and the MDA level was decreased, the differences were statistically significant (P <0.05) Significant difference (P> 0.05). IGBR can protect the liver tissue of hepatocarcinoma rats by increasing the antioxidant activity of liver tissue.