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应用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)、酶联免疫印渍试验(ELIB)和酶联免疫吸附试验(ELISA),对四种不同地理株弓形虫-CN、RH、BH、HH速殖子细胞质、细胞膜和代谢抗原组分及株间抗原差异、免疫反应性进行比较研究。ELIB实验结果:RH株高免兔血清与四株细胞质抗原反应,分别出现10、7、4、6条显色的反应区带,其中在35～38kD处都出现极明显的反应区带;与四株细胞膜抗原反应,分别出现5、6、6、9条显色反应区带,其中在31～35kD处都出现极明显的反应区带;与四株代谢抗原反应,分别出现8、7、6、7条反应区带,其中在50kD处、100～108kD处都有明显反应区带。实验结果提示,高免兔血清与四株12种抗原反应结果所出现的区带存在不同程度差异。ELISA检测免疫比值(P/N)大小比较结果表明,无论是细胞质、细胞膜或代谢抗原,在检测人弓形虫抗体阳性血清时,HH、BH较CN、RH的免疫比值高,株间抗原免疫反应似存在一定差异。
Toxoplasma gondii - CTN, RH, BH, HH Tachyzoites cytoplasm, cell membrane and metabolic antigen components and differences between the antigen, immunological reactivity were compared. ELIB results: RH rabbit serum and four cytoplasmic antigens react, respectively, there were 10,7,4,6 color reaction zone, in which 35 ~ 38kD Department were extremely obvious reaction zone; and The four cell membrane antigens reacted with 5, 6, 6 and 9 chromogenic reaction zones, respectively. At 31 ~ 35kD, the most obvious reaction zones appeared. With the reaction of the four metabolic antigens, 6,7 reaction zones, including 50kD at 100 ~ 108kD obvious reaction zone. The experimental results suggest that there are different degrees of bands appeared in the results of high immune-free rabbit serum and four strains of 12 antigens. The results of ELISA showed that the immune ratio (P / N) of HH and BH was higher than that of CN and RH in the detection of Toxoplasma gondii antibody-positive sera, both cytoplasm, cell membrane and metabolic antigen. There may be some differences.