Based on the host of tellurite glasses, the glass formation, preform manufacture, and fiber fabrication are described. The characterization of amplified spontaneous emission (ASE) from this newly fabricated single-mode Er3+-dopedtellurite fibers is also presented. When pumped at 980 nm, a very broad erbium ASE around 1.53μm was observed. The variations of ASE with fiber length and pumping power are measured and discussed. The output of 2 mW from Er3+-doped tellurite fiber ASE source was obtained under the pump power of 660 mW.