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目的探讨表皮生长因子(EGF)在神经干细胞培养中的作用。方法取新生SD大鼠神经干细胞用无血清培养技术进行培养、传代和鉴定,在传代且培养基撤除EGF后观察细胞的变化,并进行神经元特异性烯醇化酶(NSE)、神经胶质酸性蛋白(GFAP)检测,以做细胞鉴定。结果成功培养出新生大鼠神经干细胞,并进行传代,培养的神经干细胞在培养基去除EGF后能分化为神经元细胞和神经胶质细胞,EGF反应性神经干细胞主要向神经胶质细胞分化。结论新生大鼠神经干细胞能在体外适宜条件下进行长期培养、传代,且具有多向分化潜能;EGF具有促进神经干细胞快速增殖、维持神经干细胞状态、抑制神经干细胞分化的功能;EGF反应性神经干细胞具有向星形胶质细胞分化的趋势。
Objective To investigate the role of epidermal growth factor (EGF) in the culture of neural stem cells. Methods The neural stem cells of neonatal SD rats were cultured, passaged and identified by serum-free culture technique. The changes of cells were observed after subculture and EGF was removed from culture medium. Neuronal-specific enolase (NSE), glial acidity Protein (GFAP) test for cell identification. Results Neural stem cells of neonatal rats were successfully cultured and passaged. The cultured neural stem cells differentiated into neurons and glial cells after EGF was removed from the culture medium. EGF-responsive neural stem cells mainly differentiated into glial cells. Conclusion EGF can promote the proliferation of neural stem cells, maintain the status of neural stem cells and inhibit the differentiation of neural stem cells. EGF-responsive neural stem cells (NSCs) can be cultured, passaged and have multidirectional differentiation potential under appropriate conditions in vitro. Has the tendency to differentiate into astrocytes.