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目的探讨游离黏膜补片替代尿道的可行性和有效性。方法将20条成年雄性比格犬分为A、B两组进行实验。A组采用颊黏膜补片替代尿道,B组采用结肠黏膜补片替代尿道。分别取4cm×0.8cm大小的颊黏膜补片和结肠黏膜补片来替代A、B两组尿道,术后观察排尿情况,3个月后对犬行逆行尿道造影,并用F8探杆证实有无狭窄。随后处死犬测定移植物长度并作病理组织学检查。结果实验犬全部存活,除3例外均排尿通畅,A组有1例发现尿道狭窄,另有1例出现尿瘘及滴血;B组有2例发现尿道狭窄,另有2例出现尿瘘及滴血。移植物平均皱缩率A组为15.25%,B组为13.75%。移植的颊黏膜和结肠黏膜全部存活,大部分颊黏膜的鳞状上皮上已覆盖假多层的移性上皮细胞,大部分结肠黏膜的腺上皮已覆盖假多层的移性上皮细胞。结论犬的尿道黏膜可用颊黏膜补片和结肠黏膜补片来替代。
Objective To investigate the feasibility and effectiveness of free mucosal patch replacement urethra. Methods Twenty adult male Beagle dogs were divided into A and B groups for experiment. A group of buccal mucosal patch instead of the urethra, B group mucosal patch replace the urethra. The size of 4cm × 0.8cm buccal mucosa patch and colonic mucosa patch were taken to replace the urethra of group A and B respectively. The urinary excretion was observed after operation. The retrograde urethral catheterization was performed 3 months later and the presence or absence of F8 probe was used narrow. Dogs were then sacrificed to determine the length of the graft and histopathological examination. Results All the dogs survived, urination was clear except for 3 cases. In group A, urethral stricture was found in 1 case and urinary fistula and blood in 1 case. In group B, urethral stricture was found in 2 cases and urinary fistula and blood in 2 cases . The average graft shrinkage rate in group A was 15.25% and in group B was 13.75%. Transplanted buccal mucosa and colon mucosa all survived, the majority of buccal mucosa squamous epithelium has been covered with pseudostratified transplanted epithelial cells, most of the colon mucosa of the glandular epithelium has covered the pseudomembranous transplanted epithelial cells. Conclusion The urethral mucosa of dogs can be replaced by buccal mucosa patch and colonic mucosa patch.