目的　探讨粒／ 巨噬细胞集落刺激因子（ ＧＭＣＳＦ） 及白介素４（ＩＬ４） 对正常成人及肝癌患者树突状细胞（ＤＣ） 表面人白细胞抗原（ＨＬＡ）ＤＲ 及Ｂ７２ 等免疫分子表达及其免疫功能的影响。方法　以ＧＭＣＳＦ 及ＩＬ４ 联合刺激正常成人（ｎ ＝ １０） 及肝癌患者（ｎ ＝ １０）ＤＣ，检测经ＧＭＣＳＦ 及ＩＬ４ 联合刺激前后ＤＣ 表面ＨＬＡＤＲ 及Ｂ７２ 表达水平及ＤＣ 免疫功能变化。结果　经ＧＭＣＳＦ 及ＩＬ４ 联合刺激后ＤＣ 表面ＨＬＡＤＲ 及Ｂ７２ 表达水平在肿瘤患者（６－７ ±１－６ 、６－１ ±１－１ 增至１３－１ ±２－３ 、１１－４ ±２－０ＶＯＦ，Ｐ＜ ０－０１） 及正常成人（１０－７ ±１－４ 、９－６ ±１－２ 增至１４ ±２－２ 、１１ ±１－７ＶＯＦ，Ｐ＜ ０－０５） 均有增高；该ＤＣ 免疫诱导能力亦相应增强（ 肝癌患者：由３１００ ±１２０ 增至６４００ ±１４０ｃｐｍ ，Ｐ＜ ０－０１ ；正常人：由６２００ ±９０ 增至７０００ ±１１０ｃｐｍ ，Ｐ＞ ０－０５） 。结论　ＧＭＣＳＦ 及ＩＬ４ 联合刺激能增强正常成人及肝癌患者ＤＣ 表面ＨＬＡＤＲ 及Ｂ７２ 表达水平并进一步增强ＤＣ 免疫功能。提示联合应 Objective To investigate the effects of granulocyte-macrophage colony-stimulating factor (GMCSF) and interleukin4 (IL4) on the surface of dendritic cells (DCs) in normal adults and patients with hepatocellular carcinoma (HDR) and B72 Expression of immune molecules such as immune function. Methods GMCSF and IL4 were used to stimulate normal adult (n = 10) and hepatocellular carcinoma (n = 10) DCs to detect HLADR and B72 expression on DC surface before and after combined GMCSF and IL4 stimulation. Levels and changes in DC immune function. Results The expression of HLADR and B72 on the DC surface after combined stimulation of GMCSF and IL4 in tumor patients (6-7±1-6, 6-1±1-1 increased to 13-1 ±2 3,11-4 ±2-0VOF,P< 0-01) and normal adults (10-7 ±1-4,9-6 ±1-2 to 14 ±2-2,11 ±1-7VOF,P <0-05) increased; the immune induction capacity of DC increased accordingly (hepatoma patients: from 3100 ± 120 to 6400 ± 140 cpm, P <0-01; normal people: from 6200 ± 90 to 7000 ± 110 cpm, P> 0-05). Conclusion GMCSF and IL4 combined stimulation can increase the expression of HLADR and B72 on the surface of DCs in normal adults and liver cancer patients, and further enhance the DC immune function. Prompt joint