论文部分内容阅读
目的 观察并比较山莨菪碱与地塞米松对内毒素休克兔血压及血浆一氧化氮 (NO)水平的影响 .方法 采用静脉注射细菌内毒素复制内毒素休克模型 ,颈动脉 ,颈静脉插管法测定平均动脉压 (MAP)与中心静脉压 (CVP) ,用硝酸还原酶法加 Griess法测定血浆中 NO2 - 与 NO3- 的总含量间接反映 NO的水平 .结果 给予内毒素后 ,模型组的 MAP迅速下降 ,在低水平维持 ;CVP在 2 h后开始下降 ,4h后进一步下降 .山莨菪碱治疗组与地塞米松治疗组的 MAP未见明显下降 ,优于模型组 (P<0 .0 1) ;CVP无明显变化 ,与模型组差别显著 (P<0 .0 1) .注射内毒素 1h后模型组的血浆 NO生成水平进行性升高 ,相应的地塞米松治疗组 4h内未有明显变化 ,与模型组差异显著 (P<0 .0 1) .山莨菪碱治疗组的血浆 NO水平在 1h后也开始上升 (P<0 .0 5 ) ,但在 2 h时其 NO水平明显低于模型组 (P<0 .0 5 ) .结论 山莨菪碱能够明显改善内毒素休克兔的低血压状态 ,并对其 NO的生成具有抑制作用
Objective To observe and compare the effects of anisodamine and dexamethasone on the blood pressure and the level of plasma nitric oxide (NO) in rabbits with endotoxin shock.Methods The model of endotoxin shock was induced by intravenous injection of bacterial endotoxin, carotid artery and jugular vein catheterization The mean arterial pressure (MAP) and central venous pressure (CVP) were measured and the level of NO was measured indirectly by the nitrate reductase method and the Griess method.Results After the endotoxin administration, MAP CVP decreased at 2 h and further decreased at 4 h.The MAP of anisodamine group and dexamethasone group was not significantly lower than that of model group (P <0.01) ), CVP had no significant change, and the difference between the model group and the model group was significant (P <0.01) .The level of plasma NO production in model group increased progressively after 1h injection of endotoxin, and the corresponding dexamethasone treatment group had no significant within 4h (P <0.01). The plasma NO level in anisodamine treatment group also began to increase after 1h (P <0.05), but it was significantly lower at 2h In the model group (P <0.05) Conclusion Anisodamine can be significantly changed Endotoxic shock hypotension rabbit, inhibited NO production and its