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apoA-Ⅰ、apoCⅢ是北京鸭高密度脂蛋白(HDL)中的二种主要载脂蛋白(apo),研究其功能区域有助于了解它们在HDL形成与代谢中的作用机理及其与整个脂质代谢,动脉粥样硬化形成与发展的关系。本文用溴化氰(CNBr)分别将鸭apoA-Ⅰ。apoCⅢ进行裂解,所得产物用HPLC分离,发现鸭血清apoA～Ⅰ有三个主要分离峰(P_5、P6和P7)及3个很小峰;电泳鉴定示P_5,P_6各为分子量约3500和7600的单一带,P_7则含分子量约21000,16000和13000的3条带,3小峰在电泳上未鉴定出来;apoCⅢCNBr裂解产物则有四个峰(P_3,P_4,P_5和P_6),电泳鉴定P_3、P_4和P_5峰的分子量分子约1000、2500
ApoA-Ⅰ and apoCⅢ are the two major apolipoproteins in Beijing duck HDL. Studying their functional regions helps to understand their mechanism of action in the formation and metabolism of HDL and its interaction with the whole lipid Metabolism, the relationship between the formation and development of atherosclerosis. In this paper, cyanogen bromide (CNBr) duck apoA-Ⅰ. ApoC Ⅲ was cleaved. The products were separated by HPLC and found that duck serum apoA Ⅰ had three major peaks (P_5, P_6 and P_7) and three very small peaks. Electrophoresis showed that P_5 and P_6 were single bands with molecular weights of 3,500 and 7,600 , While P_7 contained 3 bands with molecular weights of about 21000, 16000 and 13000, and the 3 small peaks were not identified by electrophoresis. There were four peaks (P_3, P_4, P_5 and P_6) in the cleavage products of apoCⅢCNBr and P_3, P_4 and P_5 Peak molecular weight molecular about 1000,2500