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目的:探讨川芎嗪对局灶性脑缺血再灌注后大鼠线粒体膜流动性和线粒体膜脂质堆积密度的影响,从线粒体角度探讨其抗脑缺血再灌注损伤的作用机制。方法:将40只健康雄性Wistar大鼠随机分为4组,即假手术组、模型对照组、川芎嗪组、尼莫地平组,每组10只。建立脑缺血再灌注模型,川芎嗪组腹腔注射川芎嗪注射液40mg/kg。结果:川芎嗪组线粒体膜η,与模型组比较有显著性差异(P<0.01);川芎嗪组线粒体膜脂质堆积密度显著高于模型组(P<0.01)。结论:川芎嗪能提高大鼠脑缺血再灌注损伤时脑组织线粒体膜脂流动性,可显著提高脑缺血再灌注大鼠脑组织线粒体膜脂质堆积密度。
Objective: To investigate the effects of tetramethylpyrazine on mitochondrial membrane fluidity and mitochondrial membrane lipid packing density in rats after focal cerebral ischemia and reperfusion, and to explore its mechanism of action against cerebral ischemia-reperfusion injury from the perspective of mitochondria. Methods: Forty healthy male Wistar rats were randomly divided into four groups: sham operation group, model control group, ligustrazine group, nimodipine group, 10 rats in each group. A model of cerebral ischemia-reperfusion was established. Ligustrazine group was injected intraperitoneally with Ligustrazine injection 40 mg/kg. Results: The mitochondrial membrane η in the tetramethylpyrazine group was significantly different from that in the model group (P<0.01). The lipid accumulation density of the mitochondrial membrane in the tetramethylpyrazine group was significantly higher than that in the model group (P<0.01). Conclusion: Tetramethylpyrazine can improve the brain fluid mitochondrial membrane lipid fluidity in rats with cerebral ischemia reperfusion injury, and can significantly increase the brain tissue mitochondrial membrane lipid accumulation density after cerebral ischemia reperfusion.