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目的建立检测中兽药中金刚烷胺残留的UPLC-MS/MS方法。方法样品经提取稀释后,采用ACQUITY UPLCTMBEH C18色谱柱为分离柱,质谱正离子扫描测定。结果金刚烷胺在5~100 ng·mL-1与峰面积线性关系良好(r2=0.999 2);样品检出限为1μg·mL-1,定量限为2μg·mL-1。在40、50、60 mg·mL-1回收率为85%~115%,批内批间变异系数均<10%。结论本方法快速、灵敏、重现性好,适用于中兽药中非法添加金刚烷胺的检测。
Objective To establish a UPLC-MS / MS method for the determination of amantadine in veterinary medicine. Methods After the samples were diluted, ACQUITY UPLC TMBEH C18 column was used as the separation column for mass spectrometry positive ion scan. Results Amantadine showed a good linearity (r2 = 0.999 2) with a peak area of 5-100 ng · mL-1. The detection limit was 1 μg · mL-1 and the limit of quantification was 2 μg · mL-1. The recoveries at 40, 50 and 60 mg · mL-1 ranged from 85% to 115% and the coefficients of variation among batches were all <10%. Conclusion The method is rapid, sensitive and reproducible. It is suitable for the detection of amantadine illegally added to veterinary drugs.