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目的研究从褐藻展枝马尾藻Sargassumpatens中分离到的一种硫酸多糖SP2的抗病毒活性及抗病毒作用机理。方法通过细胞致病效应实验、MTT测定和空斑形成抑制实验对该多糖的抗病毒活性、细胞毒性和可能的抗病毒机理进行测定。结果通过空斑形成抑制实验测定结果显示,该多糖对呼吸道合胞病毒有好的抗病毒活性,其EC50值为1.1但没有抗流感病毒A和B、副流感病毒的活性。多糖的CC50高达3000μg.mL-1。其对呼吸道合胞病毒的抗病毒选择性指数被估计大于2727。用SP2预处理宿主细胞,不显示任何对病毒感染的保护作用。SP2对呼吸道合胞病毒有一定的杀病毒活性,其EC50值超过100μg.mL-1。时间加入实验证明,只有在37℃呼吸道合胞病毒吸附时期加入SP2才能完全抑制病毒的复制。结论SP2可能是一种有潜力的抗呼吸道合胞病毒感染的多糖。其对呼吸道合胞病毒的抗病毒作用机制与抑制病毒的吸附有关。
Objective To study the antiviral activity and antiviral mechanism of a sulfated polysaccharide SP2 isolated from Sargassum patens of Brown algae. Methods The antiviral activity, cytotoxicity and possible antiviral mechanism of the polysaccharide were determined by cytopathogenic effect assay, MTT assay and plaque formation inhibition assay. Results The results of plaque formation inhibition assays show that the polysaccharide has good antiviral activity against respiratory syncytial virus with an EC50 value of 1.1 but no activity against influenza viruses A and B, parainfluenza virus. The CC50 of the polysaccharide is as high as 3000 μg.mL-1. Its antiviral selectivity index for RSV is estimated to be greater than 2727. Pre-treatment of host cells with SP2 does not show any protection against viral infection. SP2 has a certain virucidal activity against respiratory syncytial virus, and its EC50 value exceeds 100 μg.mL-1. Time added experiments have shown that only by adding SP2 to the respiratory syncytial virus adsorption at 37 °C can completely inhibit the replication of the virus. Conclusion SP2 may be a potential polysaccharide against respiratory syncytial virus infection. Its antiviral action mechanism on respiratory syncytial virus is related to the inhibition of virus adsorption.