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目的:探讨膳食黑米皮对载脂蛋白E基因缺陷小鼠(apoE基因缺陷小鼠)动脉粥样硬化(AS)成的影响及其作用机制,为AS的膳食防治研究提供理论依据。方法:分离apoE基因缺陷小鼠心脏,用油红O染色法和图像分析法检测小鼠主动脉窦动脉粥样斑块面积的大小。分离小鼠血清,硝酸还原酶法检测一氧化氮(NO_2~-/NO_3~)含量;分离小鼠主动脉,抽提总蛋白,放射免疫法检测诱导型一氧化氮合酶(iNOS)的活性;抽提总RNA,用逆转录-聚合酶链式反应(RT-PCR)检测iNOS mRNA的表达。结果:黑米皮组apoE基因缺陷小鼠主动脉窦动脉粥样斑块面积显著低于阳性组和白米皮组(P<0.01)。黑米皮组与白米皮组血清中NO_2~-/NO_3~-含量并无显著差异(P>0.05);黑米皮组小鼠主动脉iNOS的活性和iNOS mRNA的表达量显著低于阳性组和白米皮组(P<0.05)。结论:膳食黑米皮可抑制apoE基因缺陷小鼠iNOS mRNA的表达,这可能是黑米皮抗AS作用的机制之一。
Objective: To investigate the effect and mechanism of dietary black rice bran on atherosclerosis (AS) induced by apolipoprotein E gene-deficient mice (apoE-deficient mice), and to provide a theoretical basis for the dietary prevention and treatment of AS. Methods: The heart of apoE-deficient mice was isolated and the size of atherosclerotic plaque in aortic sinus was detected by oil red O staining and image analysis. The serum of mice was separated and the content of nitric oxide (NO_2 ~ - / NO_3 ~) was detected by nitrate reductase method. The aorta of mice were isolated and the total protein was extracted. The activity of inducible nitric oxide synthase (iNOS) Total RNA was extracted and iNOS mRNA expression was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results: The area of aortic atherosclerosis in apoE gene-deficient mice was significantly lower than that in the positive group and the white rice skin group (P <0.01). There was no significant difference in the content of NO 2 - / NO 3 - - between the black rice skin group and the white rice skin group (P> 0.05). The activity of iNOS and the expression of iNOS mRNA of the aorta in the black rice skin group were significantly lower than those in the positive group And white rice skin group (P <0.05). Conclusion: Dietary black rice bran can inhibit iNOS mRNA expression in apoE-deficient mice, which may be one of the mechanisms of anti-AS effect of black rice bran.