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目的:为了阐明Pb~(2+)污染对延胡索的毒害机理,探索重金属对延胡索生长发育的影响。方法:采用土培实验,研究不同浓度的Pb~(2+)(0、100、200、300、500、600、700、900 mg/kg)对延胡索生长、抗氧化酶活性和Pb~(2+)积累的影响。结果:延胡索在Pb~(2+)污染下生长受抑制,生物量下降;Pb~(2+)污染能明显影响延胡索抗氧化酶活性,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性均随Pb~(2+)浓度的增加而先增后减,过氧化物酶(POD)的活性随Pb~(2+)浓度的增加而逐渐增加;Pb~(2+)污染能明显影响延胡索叶绿素含量、可溶性糖含量、丙二醛(MDA)含量,叶绿素含量与可溶性糖的含量随着Pb~(2+)浓度的增加而逐渐降低,而MDA的含量逐渐增加;随着Pb~(2+)污染浓度的增加,延胡索对Pb~(2+)的积累在地下块茎逐渐增多,在Pb~(2+)浓度为500 mg/kg时Pb~(2+)含量增加速度最大。结论:铅污染对延胡索的生长和生理特性有较大的影响,延胡索栽培中应尽量选择Pb~(2+)污染较小的土地进行。
OBJECTIVE: To elucidate the toxic mechanism of Pb ~ (2+) pollution on Corydalis yanhusuo and explore the effect of heavy metals on the growth and development of Corydalis yanhusuo. Methods: The effects of Pb 2+ (0,100,200,300,500,600,700,900 mg / kg) on the growth, antioxidant enzyme activities and Pb ~ (2) +) The impact of accumulation. Results: The growth of corydalis was inhibited under the Pb 2+ pollution and the biomass decreased. The Pb 2+ pollution could significantly affect the activities of antioxidant enzymes, superoxide dismutase (SOD) and catalase CAT) increased firstly and then decreased with the increase of Pb 2+ concentration. The activity of peroxidase (POD) increased gradually with the increase of Pb 2+ concentration. Pollution could significantly affect chlorophyll content, soluble sugar content, malondialdehyde (MDA) content, chlorophyll content and soluble sugar content of Corydalis yanuensis decreased with the increase of Pb 2+ concentration, while MDA content increased gradually With the increase of Pb2 + concentration, the accumulation of Pb2 + in the fumarate increased gradually in the underground tubers, and the content of Pb2 + increased when the concentration of Pb2 + was 500 mg / kg The fastest. CONCLUSION: Lead pollution has a great influence on the growth and physiological characteristics of Corydalis yanhusuo. In the cultivation of Corydalis yanhusuo, the soil with less Pb2 + pollution should be chosen as far as possible.