[Objective]As a mosquito-repelling ornamental plant,Pelargonium×Citrosum Vanleenii(P.× Citrosum Vanleenii)is hard to be acquired because of its hybrid background,the paper was to a new regeneration system of(P.× Citrosum Vanleenii).[Method] By studying the influence of plant growth regulators(PGRs)on explant type(leaves and petioles),the optimal combinations of PGRs to maximize SELSs(somatic embryo-like structure)and buds were established.[Result]0.2 mg/L NAA+1.0 mg/L BA was best for LS(leaves segments)and 0.2 mg/L NAA + 1.5 mg/L BAs was best for PS(petioles segments).Cultured plantlets were successfully acclimatized in soil where they grew normally without any morphological variation.Although both LS and PS were usable,the leaf was a better explant for induction of embryogenic calli,somatic embryo-like structures and buds.[Conclusion]This work offered a rapid and efficient system for plant regeneration of P.×Citrosum Vanleenii. [Objective] As a mosquito-repelling ornamental plant, Pelargonium × Citrosum Vanleenii (P. × Citrosum Vanleenii) was hard to be acquired because of its hybrid background, the paper was to a new regeneration system of (P. × Citrosum Vanleenii). [Method] By studying the influence of plant growth regulators (PGRs) on explant types (leaves and petioles), the optimal combinations of PGRs to maximize SELSs (somatic embryo-like structures) and buds were established. [Result] 0.2 mg / L NAA + 1.0 mg / L BA was best for LS (leaves segments) and 0.2 mg / L NAA + 1.5 mg / L BAs was best for PS (petioles segments). Cultured plantlets were successfully acclimatized in soil where they grew normally without any morphological variation. Although both LS and PS were usable, the leaf was a better explant for induction of embryogenic calli, somatic embryo-like structures and buds. [Conclusion] This work offers a rapid and efficient system for plant regeneration of P. × Citrosum Vanleenii .