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为了研究谷胱甘肽(GSH)对顺铂(CDDP)所致染色体畸变的影响,将昆明小鼠随机分为空白对照组、GSH组、CDDP组、GSH+CDDP组进行实验,GSH组按照1 200 mg/(kg·d)连续3天尾静脉注射GSH,对照组注射等量生理盐水;CDDP组于第2天一次注射顺铂20 mg/kg。对小鼠尾静脉取血检测血常规,处死小鼠,取骨髓进行有核细胞计数、微核实验和染色体G显带,双盲阅片并进行统计学分析。结果表明,对照组细胞染色体数目与形态完好,微核出现的机率极低。GSH组与空白对照组相比无显著差异(P>0.05)。CDDP组、GSH+CDDP组出现了明显的骨髓抑制现象,染色体畸变率及微核率显著增高,与空白对照组比较均有显著性差异(P<0.01)。GSH+CDDP组与CDDP组相比染色体畸变率明显下降(P<0.05)。表明谷胱甘肽对顺铂所致的染色体损伤具有一定的保护作用。
To investigate the effect of glutathione (GSH) on chromosome aberration caused by cisplatin (CDDP), Kunming mice were randomly divided into blank control group, GSH group, CDDP group and GSH + CDDP group. GSH was injected into the tail vein at 200 mg / (kg · d) for 3 consecutive days, while the control group received the same amount of saline. In the CDDP group, cisplatin (20 mg / kg) was injected on the second day. Blood samples were collected from the caudal vein of mice to test blood, mice were killed, bone marrow was taken for nucleated cell counting, micronucleus test and chromosome G banding, double blind reading and statistical analysis. The results showed that the number and morphology of chromosomes in the control group were intact, and the probability of occurrence of micronuclei was very low. There was no significant difference between GSH group and blank control group (P> 0.05). CDDP group and GSH + CDDP group showed obvious myelosuppression, chromosome aberration rate and micronucleus rate were significantly increased, compared with the blank control group were significantly different (P <0.01). The chromosome aberration rate of GSH + CDDP group was significantly lower than that of CDDP group (P <0.05). Glutathione shows cisplatin-induced chromosomal damage has some protective effect.