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目的 研究运动对链脲佐菌素所致的糖尿病大鼠骨骼肌细胞胰岛素受体 ,葡萄糖运载体 4(glucosetransporter,GLUT4)基因表达和蛋白含量的作用 ,探讨运动训练改善糖尿病大鼠糖代谢的可能作用机理。方法 实验大鼠分 3组 :糖尿病非运动组 ,糖尿病运动组、正常对照组。糖尿病运动组大鼠进行 6周游泳运动。用Western印迹法检测大鼠骨骼肌细胞GLUT4蛋白含量 ,用斑点印迹杂交法检测骨骼肌细胞内GLUT4mRNA含量。结果 糖尿病大鼠血糖水平明显升高 ,血胰岛素水平明显降低 ,骨骼肌细胞GLUT4蛋白含量和mRNA含量明显减少 ,与正常对照组相比分别减少 30 .7%和5 4 9%。糖尿病运动组大鼠经过 6周运动训练 ,血糖由 18.5mmol/L± 1.9mmol/L降至 14.0mmol/L±3.3mmol/L(P <0 .0 1) ,骨骼肌细胞GLUT4蛋白含量和mRNA含量较糖尿病组大鼠分别增加 6 0 .8%和5 6 % ;但运动训练并不增加糖尿病大鼠骨骼肌细胞胰岛素受体与胰岛素的结合率。结论 运动训练主要增加糖尿病大鼠骨骼肌细胞GLUT4基因表达和GLUT4蛋白含量 ,通过胰岛素受体后机制改善糖尿病大鼠外周组织对胰岛素的抵抗 ,降低血糖。
Objective To investigate the effect of exercise on the gene expression and protein content of insulin receptor and glucosetransporter (GLUT4) in skeletal muscle cells of streptozotocin-induced diabetic rats and to explore the possible effect of exercise training on glucose metabolism in diabetic rats Mechanism of action. Methods Experimental rats were divided into 3 groups: diabetic non-exercise group, diabetic exercise group and normal control group. Rats in the Diabetes Exercise group were given 6 weeks of swimming. The content of GLUT4 protein in rat skeletal muscle cells was detected by Western blotting, and the content of GLUT4 mRNA in skeletal muscle cells was detected by dot blot hybridization. Results The blood glucose level of diabetic rats increased significantly, the level of blood insulin decreased significantly, and the content of GLUT4 protein and mRNA of skeletal muscle cells decreased significantly, which were decreased by 30.7% and 54.9% respectively compared with the normal control group. The diabetic rats exercised for 6 weeks after exercise, blood glucose decreased from 18.5mmol / L ± 1.9mmol / L to 14.0mmol / L ± 3.3mmol / L (P <0.01), the content of GLUT4 protein and mRNA Compared with the diabetic group, the content increased by 60.8% and 56% respectively. However, exercise training did not increase the binding rate of insulin receptor to insulin in skeletal muscle cells of diabetic rats. Conclusion Exercise training mainly increases GLUT4 gene expression and GLUT4 protein content in skeletal muscle cells of diabetic rats. It improves insulin resistance and reduces blood sugar in the peripheral tissues of diabetic rats through the mechanism of insulin receptor.