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目的:建立兔VX2食管癌移植瘤模型,探讨其成瘤率、生长特性及转移情况,为进一步研究食管癌及寻找有效治疗方法提供平台。方法:将兔VX2肿瘤剪碎至1 mm3左右的组织块,通过外科手术将肿瘤块植入腹段食管黏膜下层,于接种后第1、2及3周分别行食管造影和CT扫描检查,观察肿瘤生长情况。3周后,处死兔子取标本行病理学检测。结果:12只兔子除2只死于手术并发症外(胃穿孔及误缝食管所致的梗阻),其余10只接种后1周,未见肿瘤生长;2周后食管造影显示食管下段充盈缺损。CT扫描示食管下段走行区局限性管壁增厚,有明显软组织肿块影,平均最大径为1.04 cm(0.7~1.5 cm);3周后兔子消瘦明显,食管造影示食管下段黏膜破坏,充盈缺损明显,4只兔子出现食管穿孔。CT扫描示食管下段软组织肿块影明显增大,平均最大径为2.28 cm(1.7~4.3 cm)。瘤体及淋巴结病理检查证实,成瘤及淋巴结转移成功率为100%。结论:采用兔VX2瘤块移植法可成功建立食管癌移植模型,且具有周期短,成瘤率高,生长迅速等特点。
OBJECTIVE: To establish a rabbit VX2 esophageal carcinoma xenograft model to investigate its tumorigenicity, growth characteristics and metastasis, to provide a platform for further study of esophageal cancer and finding effective treatment. Methods: The rabbit VX2 tumor was cut into 1 mm3 tissue blocks, and the tumor masses were implanted into the abdominal submucosa by surgery. The esophageal angiography and CT scan were performed at 1, 2 and 3 weeks after inoculation Tumor growth. After 3 weeks, the rabbits were sacrificed for pathological examination. RESULTS: Except for 2 complications, 12 rabbits died of complications of surgery (obstruction caused by perforation of gastric cavity and wrong esophagus), and no growth was observed in the other 10 rabbits after one week of inoculation. The esophageal angiography showed that the lower esophagus filling defect . The CT scan showed that the limited wall of the esophagus was thicker with obvious soft tissue mass with an average maximum diameter of 1.04 cm (0.7-1.5 cm). The rabbits were emaciated after 3 weeks. Esophageal angiography showed that the lower esophageal mucosa was damaged and the filling defect Obviously, 4 rabbits appeared esophageal perforation. CT scan revealed a significant increase in soft tissue mass in the lower esophagus, with an average maximum diameter of 2.28 cm (1.7 to 4.3 cm). Pathological examination of the tumor and lymph nodes confirmed that the success rate of tumor formation and lymph node metastasis was 100%. Conclusion: Rabbit VX2 tumor mass transplantation can successfully establish esophageal carcinoma transplantion model with short cycle, high rate of tumorigenesis and rapid growth.