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选用阿拉尔棉铃虫自然敏感种群(AL-S),在详细观察2龄中期幼虫的形态、行为特征及比较其生理学特性对药剂敏感性影响的基础上,用生理特性一致的2龄中期幼虫,测定了10种常用杀虫剂浸叶法的毒力基线。结果表明:①2龄中期幼虫的形态和行为特征为头宽≤体宽,体色不透明肉色,体节明显且行动较敏捷。此时2龄幼虫的时龄约为10～22h,体重1.33±0.27mg;②试虫生理特性差异影响对药剂的敏感性。2龄幼虫不同发育历期的敏感性,以前期最敏感,中、后期较接近;雌蛾不同蛾龄的卵发育的2龄幼虫敏感性,以4日龄蛾卵的幼虫敏感性最低,7日龄蛾卵最敏感,5、6日龄蛾的敏感性相近,且介于上述二者之间。故在测定毒力基线时,为保持试虫生理同质性,宜选用5、6日龄雌蛾卵的2龄中期幼虫为佳。③用2龄中期幼虫,获得10种药剂浸叶法的毒力基线,杀虫剂致死中浓度(LC_(50))按以下次序增大:功夫菊酯、溴氰菊酯、氰戊菊酯、硫丹、甲基E605、辛硫磷、毒死蜱、丙溴磷、久效磷、灭多威。
Based on the detailed observation of the morphological and behavioral characteristics of the second-instar larvae and the comparison of the effects of their physiological characteristics on the susceptibility to insecticides, the second-instar larvae with the same physiological characteristics were selected. The virulence baseline of 10 commonly used insecticide dipping leaves was determined. The results showed that: ① The morphological and behavioral characteristics of middle-aged 2-year-old larvae were head width ≤ body width, body color opaque flesh color, obvious body section and quick action. At this time, the second instar larvae age is about 10 ~ 22h, body weight 1.33 ± 0.27mg; ② test insects differences in physiological characteristics of the sensitivity of the agent. The sensitivity of the 2nd instar larvae to different developmental stages was the most sensitive in the early stage and the closer to the middle and late stages. The susceptibility of 2nd instar larvae to egg development of different moth age was the lowest, Day-old moth eggs are the most sensitive, 5, 6-day-old moths are similar in sensitivity, and between the two. Therefore, in the determination of virulence baseline, in order to maintain the physiological homogeneity of insects, should be used 5,6-day-old female moth eggs 2 instar larvae are better. (3) The second-instar larvae were used to get the baseline of virulence of ten kinds of agents and the lethal concentration of insecticides (LC_ (50)) increased in the order of: cyhalothrin, deltamethrin and fenvalerate , Endosulfan, methyl E605, phoxim, chlorpyrifos, profenofos, monocrotophos, methomyl.