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目的 :探讨蜜环菌多糖含药血清对Aβ25-35诱导大鼠肾上腺嗜铬细胞瘤细胞(PC12细胞)损伤保护的作用机制。方法 :在Aβ25-35诱导PC12细胞损伤模型的基础上加入蜜环菌多糖含药血清,采用比色法检测含药血清对Aβ25-35诱导PC12细胞损伤培养液中乳酸脱氢酶的活力、丙二醛含量及超氧化物歧化酶活力的影响。结果 :不同浓度的含药血清对Aβ25-35损伤的PC12细胞的存活率有提高作用,含药血清浓度在5%时达最大值(P<0.05或P<0.01);5%浓度的含药血清可明显降低模型细胞培养液中LDH活力、MDA含量,提高SOD活力。结论 :蜜环菌多糖含药血清对Aβ25-35损伤的PC12细胞有保护作用,机制可能与提高细胞抗氧化能力有关。
Objective: To investigate the protective effect of Polysaccharide from Armillaria melitensis on Aβ25-35-induced injury in adrenal pheochromocytoma cells (PC12 cells). Methods: Aβ25-35-induced PC12 cell injury model based on the addition of honey Armillaria polysaccharides containing serum, assayed by colorimetric assay Aβ25-35 PC12 cells induced injury in culture medium lactate dehydrogenase activity, C Dialdehyde content and superoxide dismutase activity. Results: The drug-containing serums of different concentrations increased the survival rate of PC12 cells damaged by Aβ25-35, with the serum concentration of 5% at maximum (P <0.05 or P <0.01) Serum can significantly reduce LDH activity in model cell culture medium, MDA content, improve SOD activity. Conclusion: The drug serum containing Armillaria polysaccharide has a protective effect on PC12 cells damaged by Aβ25-35, and its mechanism may be related to the enhancement of cell antioxidant capacity.