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目的在转录水平上研究外源性神经节苷脂（ＧＭ３）、（ＧＤ３）对小鼠巨噬细胞抗肿瘤相关因子白细胞介素１β（ＩＬ１β）、肿瘤坏死因子α（ＴＮＦα）、白细胞介素６（ＩＬ６）表达的影响。方法用反转录聚合酶链反应（ＲＴＰＣＲ）法和密度扫描半定量ＰＣＲ产物，比较测定细胞因子ＩＬ１β、ＴＮＦα、ＩＬ６ｍＲＮＡ（信使核糖核酸）表达量。结果单唾液酸神经节苷脂ＧＭ３、双唾液酸神经节苷脂ＧＤ３对巨噬细胞作用２４ｈ后，与未处理组比较，ＧＭ３可极大地增加ＩＬ１β、ＩＬ６、ＴＮＦαｍＲＮＡ的表达，分别在６ｍｇ／Ｌ、４ｍｇ／Ｌ和４ｍｇ／Ｌ时使３种细胞因子增加１３６、８３和４６４倍。ＧＤ３对ｍＲＮＡ的表达仅见微弱调节作用。结论ＧＭ３可在转录水平上对上述３种细胞因子进行正调控，且与剂量呈相关性，而ＧＤ３则基本上无作用。一定程度上反映了ＧＭ３、ＧＤ３对巨噬细胞免疫功能的影响。
Objective To study the effects of exogenous gangliosides (GM3) and (GD3) on the macrophage anti-tumor-related factors interleukin-1β (IL-1β), tumor necrosis factor-α (TNFα), leukocytes at the transcriptional level. Interleukin-6 (IL-6) expression effects. Methods Reverse transcription-polymerase chain reaction (RT-PCR) and density-scanning semi-quantitative PCR products were used to compare the expression levels of IL-1β, TNFα and IL-6 mRNA (mRNA). Results After treatment with monosialoganglioside GM3 and disialoganglioside GD3 on macrophages for 24 h, compared with the untreated group, GM3 significantly increased the expression of IL-1β, IL-6 and TNFα mRNA. At 6 mg/L, 4 mg/L, and 4 mg/L, 3 cytokines were increased by 136, 83, and 464 times. The expression of GD3 mRNA only sees weak regulation. Conclusions GM3 can positively regulate the above three cytokines at the transcriptional level, and is correlated with the dose, while GD3 has no effect. To a certain extent, the effects of GM3 and GD3 on macrophage immune function were reflected.