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制备葛根素-二甲基-β-环糊精包合物并建立其包合率测定方法。采用紫外分光光度法测定葛根素-二甲基-β-环糊精包合物中葛根素的含量,由此计算包合率。结果表明葛根素在250nm处有最大吸收,浓度在15—65μg.mL-1时与吸光度呈良好的线性关系,回归方程为:A=0.0129C+0.0313,r=0.9998(n=6);平均回收率为99.23%,RSD为0.68%;测得葛根素-二甲基-β-环糊精的包合率为96.58%,RSD为1.2%。该包合物制备和含量测定方法操作简便易行,准确可靠,得到葛根素-二甲基-β-环糊精包合物的包合率很高。
Preparation of puerarin-dimethyl-β-cyclodextrin inclusion complex and determination of its inclusion rate determination. The content of puerarin in the inclusion complex of puerarin-dimethyl-β-cyclodextrin was determined by ultraviolet spectrophotometry. The results showed that the maximum absorption of puerarin at 250nm, the concentration of 15-65μg.mL-1 and absorbance showed a good linear relationship, the regression equation was: A = 0.0129C +0.0313, r = 0.9998 (n = 6); mean The recovery rate was 99.23% and the RSD was 0.68%. The inclusion rate of puerarin-dimethyl-β-cyclodextrin was 96.58% and the RSD was 1.2%. The inclusion complex preparation and determination method is simple and easy to operate, accurate and reliable, and the inclusion rate of the puerarin-dimethyl-β-cyclodextrin inclusion complex is high.