提高稻、麦、大豆等作物蛋白含量的研究近年受到各国学者的普遍重视。但在品质育种研究中,如何从杂交后代或诱发突变群体中快速筛选出高蛋白含量的株系是一个极待解决的问题。凯氏定氮法是分析蛋白质的经典方法,但对大量样品分析筛选有困难。进口设备操作简便,但价格昂贵,不便普及。我们在大豆诱变育种研究中,三年的实践,在Pinckncy法的基础上建立了大豆粗蛋白微量快速分析法。此法操作简便,成本低、取样少、准确度和稳定性都较好,适用于大豆高蛋白株系的筛选研究。 Research on improving the protein content of crops such as rice, wheat and soybeans has received universal attention by scholars in various countries in recent years. However, in the field of quality breeding research, how to rapidly screen out the lines with high protein content from the cross of offsprings or induced mutants is a problem to be solved. Kjeldahl method is a classic method for the analysis of proteins, but it is difficult to analyze and filter a large number of samples. Imported equipment is easy to operate, but expensive and inconvenient to spread. In our research on mutagenesis of soybean, three years of practice, based on the Pinckncy method, we established a rapid method for the rapid determination of soybean crude protein. This method is simple, low cost, less sampling, accuracy and stability are good, suitable for screening high-protein soybean lines.