论文部分内容阅读
目的了解宁波地区人乳头瘤病毒高危亚型HPV58的型别分布和癌相关基因E6、E7区基因变异谱系特征。方法收集调查社区和医院就诊妇女的宫颈脱落细胞样本进行HPV分型检测,并对其中HPV58亚型阳性株进行癌相关基因测序和序列分析。结果778例社区人群样本HPV58阳性16例,检出率2.06%,1 835例就诊人群样本HPV58阳性74例,检出率4.03%;HPV58病毒株E6、E7基因序列分别成功获得6和11条,E6区有2个位点发生碱基颠换:C307T和A388C,E7区有5个位点发生碱基颠换:G694A、T726C、T744G、G761A和T803C;系统进化树分析显示宁波地区HPV58变异株主要位于该亚型基因变异谱系A的A1和A2分支。结论宁波人群HPV58感染率较高,多数感染者携带病毒的癌相关基因发生变异,应加强对人群HPV感染及其持续感染状况的监测,尤其是HPV58阳性人群。
Objective To understand the type distribution of high risk HPV58 in human papillomavirus in Ningbo and the genealogical characteristics of E6 and E7 in cancer related genes. Methods HPV types were detected in cervical exfoliated cells of women who visited community and hospital, and the HPV58 subtype positive strains were sequenced and sequenced. Results The positive rate of HPV58 in 16 samples of 778 community samples was 2.06%, and HPV58 positive samples from 1 835 outpatients were detected in 74 samples with the detection rate of 4.03%. The sequences of HPV58 E6 and E7 were 6 and 11, There were two transversions in E6 region: C307T and A388C, and transposition of base at 5 sites in E7 region: G694A, T726C, T744G, G761A and T803C. Phylogenetic analysis showed that HPV58 in Ningbo Mainly in the A1 and A2 branches of this subgenotype A lineage. Conclusion The prevalence of HPV58 in Ningbo population is high, and the majority of those infected with the virus carry mutations in the cancer-associated genes. HPV infection and the status of persistent infection in the population should be strengthened, especially in the HPV58-positive population.