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目的测定杜仲叶中4种有效成分的含量。方法采用HPLC测定绿原酸、槲皮素、山奈酚、芦丁的含量,色谱柱为Wonda Sil C_(18)Superb色谱柱(4.6 mm×250 mm,5μm),流动相:0.4%磷酸溶液(A)-甲醇(B)进行梯度洗脱,流速1 ml/min,进样量10μl,检测波长262 nm。结果绿原酸的质量在0.825~4.123μg(R2=0.9999)范围内呈良好线性关系,平均回收率为101.89%,RSD为0.49%;槲皮素的质量在0.165~0.824μg(R2=0.9998)范围内呈良好线性关系,平均回收率为99.88%,RSD为2.64%;山奈酚的质量在0.021~0.104μg(R~2=0.9999)范围内呈良好线性关系,平均回收率为100.0%,RSD为0.89%;芦丁的质量在0.002~0.012μg(R~2=0.9991)范围内呈良好线性关系,平均回收率为100.0%,RSD为0%。结论本方法简便、快速、准确、重复性好,适用于杜仲叶中各成分的含量测定。
Objective To determine the content of four active ingredients in Eucommia ulmoides leaves. Methods The contents of chlorogenic acid, quercetin, kaempferol and rutin were determined by HPLC on a Wonda Sil C_ (18) Superb column (4.6 mm × 250 mm, 5 μm) with a mobile phase of 0.4% phosphoric acid solution A) - methanol (B) gradient elution, flow rate 1 ml / min, injection volume 10μl, detection wavelength 262 nm. Results The mass of chlorogenic acid showed a good linearity in the range of 0.825 ~ 4.123μg (R2 = 0.9999) with the average recovery of 101.89% and RSD of 0.49%. The mass of quercetin was 0.165 ~ 0.824μg (R2 = 0.9998) The average recovery was 99.88%, RSD was 2.64%. The quality of kaempferol showed a good linearity within the range of 0.021-0.104μg (R ~ 2 = 0.9999) with the average recovery of 100.0%. The RSD 0.89%. The quality of rutin showed good linearity in the range of 0.002 ~ 0.012μg (R ~ 2 = 0.9991) with the average recovery of 100.0% and RSD of 0%. Conclusion The method is simple, rapid, accurate and reproducible. It is suitable for the determination of each component in Eucommia leaf.