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1内皮祖细胞的来源、表面标志、分离方法内皮组细胞(endothelial progenitor cells,EPCs)的来源主要包括人脐静脉血、成人外周血、骨髓,并且发现外周血中的EPCs起源于骨髓,而脐血中的EPCs起源于胎儿的肝脏。迄今为止,EPCs尚无统一的鉴定标准,一般将表达CD34/CD133/KDR(VEGFR2或Flkl)这3种基因标志物的细胞定义为EPCs。EPCs的分离方法很多,最常见的有密度梯度离心法配合直接贴壁筛选法,除此以外还有细胞分离装置、免疫磁珠分
1 Sources, surface markers and isolation methods of endothelial progenitor cells The sources of endothelial progenitor cells (EPCs) mainly include human umbilical cord blood, adult peripheral blood and bone marrow, and found that EPCs in peripheral blood originated from bone marrow, while umbilicus EPCs in the blood originate in the fetus’s liver. To date, there is no uniform standard for EPCs. Cells that express the three genetic markers CD34 / CD133 / KDR (VEGFR2 or Flk1) are generally defined as EPCs. There are many methods for the separation of EPCs, the most common are density gradient centrifugation with direct adherent screening method, in addition to cell separation device, immunomagnetic beads