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目的研究结直肠癌患者外周血角蛋白20(cytokeratin20,CK20)mRNA的变化,探讨腹腔镜在结直肠癌手术治疗中的应用价值。方法采用反转录-聚合酶链(RT-PCR)法检测37例行根治手术治疗的结直肠癌患者和8例非恶性病变腹部手术患者的外周血CK20mRNA的表达。结果8例良性病变腹部手术患者外周血CK20mRNA无明显表达。37例结直肠癌患者总的CK20mRNA阳性表达率为39.6%(44/111),且术前、术中和术后CK20mRNA的阳性表达率比较差异无统计学意义(P>0.05)。16例开腹手术组和21例腹腔镜组术前、术中、术后相比,各组间CK20mRNA阳性表达率差异亦无统计学意义(P>0.05)。结论开腹手术和腹腔镜手术2种手术方式对CK20mRNA在结直肠癌患者的外周血中的表达差异无统计学意义。
Objective To study the changes of cytokeratin 20 (CK20) mRNA in patients with colorectal cancer and to explore the value of laparoscopy in the surgical treatment of colorectal cancer. Methods The expression of CK20mRNA in peripheral blood of 37 patients with colorectal cancer who underwent radical operation and 8 patients with non-malignant lesion underwent abdominal surgery. The expression of CK20mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results There was no significant expression of CK20mRNA in peripheral blood of 8 patients with benign lesions undergoing abdominal surgery. The positive rate of CK20 mRNA in 37 patients with colorectal cancer was 39.6% (44/111). There was no significant difference in CK20 mRNA expression between preoperative, intraoperative and postoperative (P> 0.05). There was no significant difference in the positive expression rate of CK20 mRNA between the 16 cases of laparotomy group and 21 cases of laparoscopic group before, during and after operation (P> 0.05). Conclusions There are no significant differences in the expression of CK20mRNA in the peripheral blood of patients with colorectal cancer after open surgery and laparoscopic surgery.