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Diphtheria toxoid was denatured in O.1M NH4Cl-NH3H2O basal solution with pH 9.4,yielding a cathodic and an anodic polarographic peaks at -1.52 and -1.45V(vs.SCE),respectively.The cathodic and anodic peak heights varied linearly with diphtheria toxoid concentration over the range of 2.5 to 180μg/ml by single sweep polarography,and diphtheria toxoid even as low as 0.3μg/ml could be detected by differential pulse polarography.The decreased peak current value of diphtheria toxoid due to immunochemical binding reaction was proportional to diphtheria antitoxin in the range of 2.5×10 to 5×10IU/ml,which determined diphtheria antitoxin level homogeneously.The assays are sensitive,specific,reproducible and simple in operation.The anodic peak was appl.ied to the determination of diphtheria toxoid in the triple vaccine.
Diphtheria toxoid was denatured in O.1M NH4Cl-NH3H2O basal solution with pH 9.4, yielding a cathodic and anodic polarographic peaks at -1.52 and -1.45V (vs.SCE), respectively. The cathodic and anodic peak heights varied linearly with diphtheria toxoid concentration over the range of 2.5 to 180 μg / ml by single sweep polarography, and diphtheria toxoid even as low as 0.3 μg / ml could be detected by differential pulse polarography. decreased BY current value of diphtheria toxoid due to immunochemical binding reaction was proportional to diphtheria antitoxin in the range of 2.5 × 10 to 5 × 10 IU / ml, which determined diphtheria antitoxin level homogeneously. These assays are sensitive, specific, reproducible and simple in operation. The anodic peak was appl .ied to the determination of diphtheria toxoid in the triple vaccine.