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用鼻咽癌ＣＮＥ－２Ｚ细胞系行裸小鼠皮下移植，通过间接免疫酶标和间接免疫荧光方法，观察肿瘤移植后不同时间纤维粘连蛋白（ｆｉｂｒｏｎｅｃｔｉｎ，ＦＮ）、层粘连蛋白（ｌａｍｉｎｉｎ，ＬＮ）和Ｗ型胶原（ｔｙｐｅⅣｃｏｌｌａｇｅｎ，Ⅳｃ的分布，结果：１）ＦＮ的分布方式随肿瘤移植后时间的延长，从瘤巢间的散在点状或片状布分变为瘤巢间的纤维组织内和血管周围成纤维细胞增生区域的丝状或网状分布；２）ＬＮ和Ⅳ型胶原的分布方式基本一致，从肿瘤间质中血管壁和基底膜上的连续细线状分布变为不规则连续粗线状多层分布。而在肿瘤细胞侵袭部位的血管基底膜处则表现为ＬＮ和Ⅳ型胶原消失提示：在肿瘤发展过程中的不同时期，细胞外基质蛋白的分布存在差异，细胞外基质的分布及其合成和降解的变化在肿瘤的生长进程中具有一定的作用。
The nasopharyngeal carcinoma CNE-2Z cell line was subcutaneously transplanted in nude mice. The expression of fibronectin (FN), laminin (LN) and fibronectin were detected by indirect immunofluorescence enzyme-linked immunosorbent assay and indirect immunofluorescence. And typeⅣcollagen (IVc distribution, the results: 1) FN distribution with the extension of time after tumor transplantation, scattered from the nests of interstitial or sheet-like distribution of interstitial fibrosis in the tumor tissue and Fibroblasts around the perivascular fibroblast hyperplasia; 2) The distribution of LN and type IV collagen was basically the same, from continuous thin-line distribution of vascular wall and basement membrane in tumor stroma to irregular continuous Thick line multi-layer distribution. However, the disappearance of LN and type IV collagens at the vascular basement membrane invasion site suggests that there are differences in the distribution of extracellular matrix proteins, the distribution of extracellular matrix and its synthesis and degradation during different stages of tumor development The changes in the process of tumor growth has a certain role.