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肝素钠的效价测定,全国各生化药厂对出口产品大都用羊血浆法。此法操作方便、计算简捷,其测定结果与兔全血法十分接近。美国药典一直采用此法为肝素钠效价的法定方法。在此法测定中,关键是羊血浆的制备。羊血浆制备的好坏直接影响结果测定,血浆制备不好,甚至可导致检验无法进行。血浆的制备并没有十分严格的条件控制,各地可根据各自的具体情况而定。凡是复钙(重钙化)试验时在5分钟内凝固的血浆均可用于测定。我厂在制备羊血浆的过程中有些体会,供各单位参考。从绵羊采血以分离颈动脉,插管直接放血为好。要预先在盛放血液的容器中加入8％柠
Heparin sodium titer determination, the country’s biochemical pharmaceutical companies on the export products mostly sheep plasma method. This method is easy to operate, the calculation is simple, the measurement results and rabbit whole blood method is very close. The United States Pharmacopoeia has been using this method as the legal method of heparin titer. Determination of this method, the key is the preparation of sheep plasma. The quality of goat plasma preparation directly affects the determination of the results, poor plasma preparation can even lead to the test can not be carried out. The preparation of plasma is not very strict control of the conditions, depending on the specific circumstances of each may be. Plasma coagulation within 5 minutes at any time during the re-calcium (re-calcification) test may be used for the assay. I plant some experience in the process of preparing sheep plasma for reference for all units. Blood from sheep to separate the carotid artery, intubation direct bleeding as well. To advance the container filled with blood 8% lemon