以酪蛋白为底物,采用福林—酚法对鲤鱼肝胰脏和肠道蛋白酶活性进行了研究。结果表明,鲤鱼肝胰脏和肠道内蛋白酶活性有随体重的增加而下降的趋势。比酶活在后肠最高,中肠、前肠和肝胰脏依次降低。在底物酪蛋白浓度为60～400ug/ml时,肝胰脏和肠道的最大比酶活分剐为240ug/ml和360ug/ml;在pH为5.6～8.0时,最大比酶活分别在pH7.2和pH7.6。 Casein was used as a substrate to study the protease activity of hepatopancreas and intestine of carp using the Forint-Phenol method. The results showed that carp liver, pancreas and intestine protease activity with the body weight increases with a downward trend. Specific activity in the hindgut highest, midgut, foregut and hepatopancreas decreased in turn. When the substrate casein concentration was 60-400ug / ml, the maximal specific enzyme activities of hepatopancreas and intestine were 240ug / ml and 360ug / ml respectively. When the pH was 5.6-8.0, pH 7.2 and pH 7.6.