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限制性内切酶诱导整合（ＲｅｓｔｒｉｃｔｉｏｎＥｎｚｙｍｅ－ｍｅｄｉａｔｅｄＩｎｔｅｇｒａｔｉｏｎ，简称ＲＥＭＩ，下同）已被成功用于创造随机插入突变和提高转化频率。用限制酶消化的线状质粒ｐＣＳＮ４３或ｐＢＦ１０１，并加一定量的同种限制酶，转化稻瘟病菌（Ｍ．ｇｒｉｓｅａ）的原生质体后，得到约４５０个转化子。对一些表型进行测试后，获得两个分生孢子形态突变体。与野生型相比较，突变体的分生孢子细而且长，呈棒形。ＲＥＭＩ非常有用，因其能提高转化频率，较容易地获得期望数目的转化子；限制性内切酶可随机切割寄主染色体，便于插入线状质粒，导致基因突变。可根据异常表型判定基因的功能，根据插入质粒克隆该基因。
Restriction Enzyme-mediated Integration (REMI, the same below) has been successfully used to create random insertional mutations and improve the frequency of transformation. Approximately 450 transformants were obtained by transformation of the protoplasts of M. grisea with the restriction enzyme digested linear plasmid pCSN43 or pBF101 and the addition of an amount of the same restriction enzyme. After some phenotypes were tested, two conidial morphological mutants were obtained. Compared with the wild type, the conidia of the mutant were thin and long, with a rod shape. REMI is very useful because it increases the frequency of transformation and makes it easier to obtain the desired number of transformants. Restriction endonucleases can randomly cut host chromosomes and facilitate the insertion of linear plasmids leading to gene mutations. The function of the gene can be judged according to the abnormal phenotype, and the gene is cloned according to the inserted plasmid.