L-Lysine was produced by a microbial process utilizing a Corynebacterium glutamicum (ATCC 21799) strain. L-Lysine was purified from the cultivated medium by fixed-bed and simulated moving bed (SMB) chromatography. The separation conditions including pH, eluent concentration and Lys~+ and Lys~(2+) adsorption isotherms were studied in batch adsorption. The column capacity, eluent flow rate and eluent concentration have been studied in fixed-bed chromatography. Maximum purification rate of lysine was obtained as 0.066 (g/(g·h)) (per gram resin and per hour) at an eluent flow rate of 10 (mL/min) in fixed-bed chromatography. The results obtained from SMB were 0.11 (g/(g·h)) for L-lysine purification rate and 96% for L-lysine recovery. L-Lysine was produced by a microbial process utilizing a Corynebacterium glutamicum (ATCC 21799) strain. L-Lysine was purified from the cultivated medium by fixed-bed and simulated moving bed (SMB) chromatography. The separation conditions including pH, eluent concentration and Lys ~ + and Lys ~ (2+) adsorption isotherms were studied in batch adsorption. The column capacity, eluent flow rate and eluent concentration have been studied in fixed-bed chromatography. Maximum purification rate of lysine was obtained as 0.066 (g / g · h) per gram resin and per hour at an eluent flow rate of 10 (mL / min) in fixed-bed chromatography. The results obtained from SMB were 0.11 (g / (g · h)) for L- lysine purification rate and 96% for L-lysine recovery.