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用流式细胞仪、光镜、扫描电镜和透射电镜观察单纯放射损伤（单放组）、单纯烧伤（单烧组）和放射复合伤（复合伤组）小鼠骨髓基质细胞的细胞周期、ＤＮＡ含量和形态变化。结果：①单烧组骨髓基质细胞的Ｇ２＋Ｍ期比例、ＤＮＡ含量由高到低；单放组和复合伤组Ｇ２＋Ｍ期比例、ＤＮＡ含量则由低到高；②与正常组骨髓基质细胞相比，各损伤组均出现不同特征的形态学变化；③单放组的内质网扩张，线粒体基质变淡，嵴消失；单烧组的线粒体增多、肿胀；放射复合伤的线粒体重度退行性变，结构紊乱。提示：放射复合伤骨髓基质细胞的细胞周期比例、ＤＮＡ含量和形态的改变可能是导致放射复合伤造血微环境损伤的重要原因之一。
The cell cycle of bone marrow stromal cells were detected by flow cytometry, light microscopy, scanning electron microscopy and transmission electron microscopy. The changes of cell cycle, DNA synthesis and apoptosis in bone marrow stromal cells of mice with radiation injury (single radiotherapy group), simple radiotherapy injury group (single radiotherapy group) Content and morphological changes. Results: (1) The ratio of G2 + M phase and the content of DNA in bone marrow stromal cells of single-burn group were from high to low; the proportion of G2 + M phase and the content of DNA in single-group and complex-injury group were from low to high; ②Compared with normal bone marrow stromal cells, There were morphological changes with different characteristics in each injury group. (3) The endoplasmic reticulum of the rats in the single group was dilated, the mitochondrial matrix became lighter and the ridges disappeared. The mitochondria in the single burn group increased and swollen. The mitochondria in the single burn group had severe degenerative changes. disorder. It is suggested that the ratio of cell cycle, DNA content and morphological changes of radiation-injured bone marrow stromal cells may be one of the important causes of hematopoietic microenvironment damage of radiation combined injury.