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目的检测垂体腺瘤中半乳糖凝集素-3(Gal-3)、Bcl-2和BaxmRNA和蛋白的表达水平,探讨Gal-3和凋亡相关基因Bcl-2、Bax的内在联系。方法RT-PCR检测20例垂体腺瘤Gal-3、Bcl-2和Bax mRNA的表达;免疫组化染色检测78例垂体腺瘤中Gal-3、Bcl-2和Bax蛋白表达,分析侵袭性和非侵袭性垂体腺瘤中Gal-3、Bcl-2和Bax表达的差异及其相关关系。结果侵袭组垂体腺瘤Gal-3和Bcl-2mRNA和蛋白的表达水平较非侵袭组显著增高,而Bax mRNA和蛋白在两组中的表达水平差异无显著性意义。等级相关分析显示Gal-3蛋白与Bcl-2蛋白之间有正相关关系(r=0.343,P=0.002);Gal-3蛋白与Bax蛋白之间无相关关系(r=-0.170,P=0.136);Bcl-2蛋白与Bax蛋白表达之间呈负相关关系(r=-0.292,P=0.009)。结论Gal-3可能主要是通过与Bcl-2协同作用,发挥其抗凋亡效应,相对促进垂体腺瘤细胞增殖。
Objective To detect the expression of Gal-3, Bcl-2 and Bax mRNA and protein in pituitary adenomas, and to explore the relationship between Gal-3 and apoptosis-related genes Bcl-2 and Bax. Methods RT-PCR was used to detect the expression of Gal-3, Bcl-2 and Bax in 20 cases of pituitary adenomas. The expressions of Gal-3, Bcl-2 and Bax in 78 cases of pituitary adenomas were detected by immunohistochemical staining. The difference of the expression of Gal-3, Bcl-2 and Bax in non-invasive pituitary adenomas and their correlation. Results The expression of Gal-3 and Bcl-2 mRNA and protein in pituitary adenomas of invasive group was significantly higher than that of non-invasive group, while the expression of Bax mRNA and protein in the two groups had no significant difference. There was a positive correlation between Gal-3 protein and Bcl-2 protein (r = 0.343, P = 0.002). No correlation was found between Gal-3 protein and Bax protein (r = -0.170, P = 0.136 ). There was a negative correlation between Bcl-2 protein and Bax protein expression (r = -0.292, P = 0.009). Conclusion Gal-3 may exert its anti-apoptotic effect mainly through the synergistic effect with Bcl-2, which may promote the proliferation of pituitary adenoma cells.