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目的:探讨14-3-3ζ对肺癌细胞转移的押制作用。方法:以人肺巨细胞癌低转移细胞株为模型,利用RNAi技术研究14-3-3ζ被干涉后肿瘤细胞恶性表型的变化,探讨14-3-3ζ与肿瘤转移的相关性。结果:本研究发现14-3-3ζ被干涉后细胞的集落形成能力明显提高,黏附能力明显降低,细胞迁移、侵袭能力明显提高。结论:14-3-3ζ可能抑制肺癌细胞的转移。
Objective: To investigate the escorting effect of 14-3-3ζ on lung cancer cell metastasis. Methods: Human lung giant cell carcinoma cell line with low metastasis was used as a model to study the change of malignant phenotype of 14-3-3ζ after interference with 14-3-3ζ by using RNAi technique to explore the correlation between 14-3-3ζ and tumor metastasis. Results: In this study, we found that 14-3-3ζ interference significantly increased colony formation ability, adhesion was significantly reduced, cell migration, invasion ability was significantly improved. Conclusion: 14-3-3ζ may inhibit the metastasis of lung cancer cells.