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To study transforming growth factor β1 (TGF β1) autopro duction in keloid fibroblasts and the regulation effect of blocking TGF β intr acellular signaling on rhTGF β1 autoproduction. Methods: Keloid fibroblasts cultured in vitro were treated with either rhTGF β1 (5 ng/ml) or recombinant adenovirus containing a truncated ty pe II TGF β receptor gene (50 pfu/cell). Their effects of regulating gene expr ession of TGF β1 and its receptor I and II were observed with Northern blot.Results: rhTGF β1 up regulated the gene expression of TGF β1 and receptor I, but not receptor II. Over expression of the truncated recep tor II down regulated the gene expression of TGF β1 and its receptor I, but n ot receptor II.Conclusions: TGF β1 autoproduction was observed in keloid fib roblasts. Over expression of the truncated TGF β receptor II decreased TGF β1 autoproduction via blocking TGF β receptor signaling.
To study transforming growth factor β1 (TGF β1) autopro duction in keloid fibroblasts and the regulation effect of blocking TGF β intr acellular signaling on rhTGF β1 autoproduction. Methods: Keloid fibroblasts cultured in vitro were treated with either rhTGF β1 (5 ng / ml) Their effects of regulating gene expr ession of TGF β1 and its receptor I and II were observed with Northern blot. Results: rhTGF β1 up regulated the gene (50 pfu / cell) expression of TGF β1 and receptor I, but not receptor II. Over expression of the truncated recep tor II down regulated the gene expression of TGF β1 and its receptor I, but n ot receptor II. Conclusions: TGF β1 autoproduction was observed in keloid fib roblasts. Over expression of the truncated TGF β receptor II decreased TGF β1 autoproduction via blocking TGF β receptor signaling.