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目的:探讨维生素D结合蛋白(DBP)基因rs7041位点多态性与汉族人群肥胖易感性的关系。方法:采用PCR-RFLP技术检测211例肥胖者和213例体重正常者的DBP基因rs7041位点基因型,采用logistic回归分析基因多态性与肥胖的关联性,采用多因素降维法分析基因-环境之间的交互作用。结果:肥胖组和对照组DBP基因rs7041位点TG+GG和TT基因型分布频率差异有统计学意义(χ2=5.425,P=0.020),肥胖组rs7041位点等位基因G分布频率高于对照组(χ2=5.175,P=0.023)。调整相关影响因素后,DBP基因rs7041位点TG基因型携带者肥胖危险性升高(调整OR=1.528,95%CI=1.017~2.295),TG+GG基因型携带者肥胖危险性升高(调整OR=1.605,95%CI=1.079~2.389)。进一步分析显示,DBP基因与环境因素(吸烟、饮酒、体力活动等)之间无交互作用(P>0.05)。结论:DBP基因rs7041位点多态性与肥胖易感性之间存在关联。
Objective: To investigate the relationship between rs7041 polymorphism of vitamin D binding protein (DBP) gene and susceptibility to obesity in Han population. Methods: PCR-RFLP was used to detect the rs7041 genotype of DBP gene in 211 obese and 213 normal-weight subjects. Logistic regression was used to analyze the association between gene polymorphism and obesity. The multivariate dimensionality- Interactions between environments. Results: The distribution frequencies of TG + GG and TT genotypes at rs7041 of DBP gene in obese and control groups were significantly different (χ2 = 5.425, P = 0.020). The distribution frequency of allele G at rs7041 in obesity group was higher than that in control group Group (χ2 = 5.175, P = 0.023). After adjustment for the related factors, the risk of obesity was increased in TG genotypes at rs7041 in DBP (OR = 1.528, 95% CI = 1.017-2.295), and increased risk of obesity in TG + GG carriers OR = 1.605, 95% CI = 1.079-2.389). Further analysis showed that there was no interaction between DBP gene and environmental factors (smoking, drinking, physical activity, etc.) (P> 0.05). Conclusion: There is a correlation between DBP gene rs7041 polymorphism and susceptibility to obesity.