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本文观察了灵芝对大鼠胶原蛋白性关节炎的作用。Ⅱ型胶原蛋白（ＣⅡ）１.５ｍｇ／只ｄ_０背部皮内注射，引发胶原蛋白性关节炎（ＣＩＡ）。酶联免疫吸附法测定血清抗 ＣⅡ抗体水平；［３Ｈ］－ＴｄＲ掺入法测定鼠腹腔巨噬细胞产生白细胞介素－１（ＩＬ－１）的活性；四甲基偶氮唑盐（比色法）测定鼠脾淋巴细胞培养上清中白细胞介素－２（ＩＬ－２）的活性。观测针对ＣＩＩ的皮肤迟发型变态反应和大鼠后足足肿改变水平。灵芝０.５ ｇ·ｋｇ~(－１)和１.０ ｇ·ｋｇ·ｄ~(－１)可抑制迟发型变态反应，灵芝１.０ ｇ·ｋｇ~(－１)·ｄ~(－１)可降低血清抗ＣⅡ抗体水平，恢复ＩＬ－２产生水平，但对大鼠后足足肿以及白细胞介素－１（ＩＬ－１）产生无明显改善作用。提示灵芝作为免疫调节剂，对大鼠ＣＩＡ的抗炎效果较弱，但对免疫异常指标具有改善作用。
This article observed the effect of Ganoderma lucidum on collagen-induced arthritis in rats. Type II collagen (CII) 1.5 mg/d_0 dorsal intradermal injection, induced collagenous arthritis (CIA). Serum anti-CII antibody levels were determined by enzyme-linked immunosorbent assay; [3H]-TdR incorporation assay was used to determine the activity of interleukin-1 (IL-1) produced by murine peritoneal macrophages; tetramethyl azozolium salt (colorimetric) Method) Determination of interleukin-2 (IL-2) activity in culture supernatant of mouse spleen lymphocytes. Observed changes in the level of skin delayed hypersensitivity to CII and hind foot and foot swelling in rats. Ganoderma lucidum 0.5 g·kg -1 and 1.0 g·kg·d -1 inhibited delayed-type hypersensitivity, Ganoderma lucidum 1.0 g·kg -1 ·d~(- 1) Serum anti-CII antibody levels can be reduced and IL-2 production levels restored, but there is no significant improvement in hind foot swelling and interleukin-1 (IL-1) production in rats. It is suggested that Ganoderma lucidum as an immunomodulatory agent has weaker anti-inflammatory effects on rat CIA, but it has an improved effect on immune abnormalities.