论文部分内容阅读
用人工重组的白介素 6 (IL 6 )多次免疫兔和豚鼠 ,获取高效价的IL 6抗体 .用氯氨T法制备12 5I标记IL 6 ,经SephadexG 2 5纯化 ,抗原抗体反应采用平衡一步法 ,4℃温育 2 4h后经PR试剂分离结合和游离的标记抗原 .该法测定范围 0 1～ 3 2 μg/L最低检出量为 0 lμg/L ,批内和批间误差分别小于 6 4%和 10 % .健康男性115例血清IL 6含量为 (0 2 7± 0 13) μg/L .女性 10 1例血清IL 6为 (0 2 6± 0 10 ) μg/L .男女无差异 .此外 ,用该法检测兔失血性休克再灌注损伤后 2 4h血清IL 6水平明显升高 .失血性休克大鼠淋巴液中IL 6水平明显上升 ,经山莨菪碱 (lmg/kg)治疗休克后IL 6又明显下降 .内毒素同人牙周纤维细胞共同培养不同时间也促进IL 6释放并明显高于对照水平
Rabbit and guinea pigs were immunized repeatedly with interleukin-6 (IL-6) by artificial recombination to obtain high titer IL6 antibody.125I-labeled IL6 was prepared by chloramine T method and purified by SephadexG2 5. The antigen-antibody reaction was performed by one-step equilibrium method , Incubated at 4 ℃ for 24 hours and then separated and labeled by PR reagent and free labeled antigen.The detection limit of the method was 0 l ~ 32 μg / L, the detection limit was 0 lμg / L, 4%, and 10% respectively.Serum IL-6 level in healthy male was (0 2 7 ± 0 13) μg / L in 115 cases.The level of serum IL 6 was (0 2 6 ± 0 10) μg / L in 10 1 female. .In addition, IL-6 levels in 24 h after hemorrhagic shock-reperfusion injury were significantly increased in this method.IL 6 levels in lymph of hemorrhagic shock rats were significantly increased, and shock treated with anisodamine (lmg / kg) After IL 6 significantly decreased endotoxin with human periodontal fibroblasts co-cultured at different times also promote IL 6 release and was significantly higher than the control level