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Alfin-like PHD finger是植物中特有的一类转录调控因子,在调控植物生长发育、非生物胁迫响应及抗病反应等过程中起重要作用.为全面了解Alfin-like PHD finger基因家族在雷蒙德氏棉和亚洲棉基因组中的数目、分布情况及家族各成员间的关系,进而研究和揭示Alfin-like PHD finger在棉花抵抗逆境胁迫中的作用.运用生物信息学的方法在雷蒙德氏棉(D5)和亚洲棉(A2)全基因组中分别鉴定出12个和10个Alfin-like PHD finger基因家族成员,并对家族成员的基因结构、染色体定位、保守结构域及进化关系等方面进行分析.结果表明,雷蒙德氏棉和亚洲棉中Alfin-like PHD finger转录因子具有高度保守的DUF3594结构域和PHD结构域,两个二倍体棉花中该家族基因成员之间为一对一关系;雷蒙德氏棉中的12个成员基因分布在5条染色体上,亚洲棉中的10个成员基因也分布在5条染色体上;亚细胞定位预测发现,该家族的基因分别定位于细胞核、叶绿体基质、过氧化物酶体和细胞质基质;根据结构域差异和系统发育分析结果,将Alfin-like PHD finger转录因子家族分为3个进化分支.上述结果为分离鉴定棉花中新的逆境胁迫响应基因提供参考.“,”Alfin-like PHD finger protein encodes a small specific type of plant transcription regulators that are involved in plant growth and development,abiotic stress response and disease resistance.This study was to fully understand the number and distribution and the relationships of Alfin-like PHD finger family among Gossypium arboretum (A2) and Gossypium raimondii (D5).Moreover,the influence of the gene on abiotic stress response were revealed.12 and 10 Alfin-like PHD finger proteins were identified from the diploid cotton Gossypium arboretum (A2) and Gossypium raimondii (D5) genome database.The gene structure,chromosome location,conserved domains and evolutionary relationships were analyzed.The result showed the putative proteins of these genes contained highly conserved PHD motif and DUF3594 domain.The members of Gossypium arboretum and Gossypium raimondii were one to one relationships.In addition,12 gene members of D5 cotton were distributed on 5 chromosomes,and 10 members of A2 cotton were also located on 5 chromosomes.It was found that the genes of the family were localized in the nucleus,chloroplast stroma,peroxisome and cytoplasmic matrix respectively.These proteins could be classified into 3 subgroups according to their structure and phylogenic relationship.These results might provide references for the isolation and identification of the abiotic stress responsive genes in cotton.