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为了研究铝胁迫下水稻质膜H~+-ATPase对硝态氮吸收影响,以‘滇优35号’(粳稻)为试验材料,采用溶液培养法研究50μmol·L~(-1) Al~(3+)胁迫下添加质膜H~+-ATPase的激活剂壳梭孢素(FC)和抑制剂腺苷-5’-单磷酸(AMP)对质膜H~+-ATPase水解活性和H~+-泵活性,质膜H~+-ATPase和14-3-3蛋白的相互作用对水稻NO_3~--N吸收的影响。结果表明,AMP处理使铝胁迫下水稻质膜H~+-ATPase水解活性、H~+-泵的活性,质膜H~+-ATPase和14-3-3蛋白的相互作用下降,H_2O_2和MDA含量增加,从而导致水稻NO_3~--N吸收降低。FC处理可使铝胁迫下水稻质膜H~+-ATPase水解活性、H~+-泵的活性以及质膜H~+-ATPase和14-3-3蛋白的相互作用增加,H_2O_2和MDA含量下降,从而使水稻NO_3~--N吸收增加。这些研究结果表明铝胁迫通过影响质膜H~+-ATPase与14-3-3蛋白互作来影响质膜H~+-ATPase水解活性和氢泵活性以及H_2O_2和MDA含量,进而影响对硝态氮的吸收。
In order to study the effect of plasma membrane H + -ATPase on NO3-N absorption under aluminum stress, the effects of 50 μmol·L -1 Al ~ (-1) H + -ATPase activity and plasma membrane H + -ATPase activity were increased by the addition of the cytosolic sepsis (FC) and adenosine 5’-monophosphate (AMP) Effect of Pump Activity, Plasma Membrane H ~ + -ATPase and 14-3-3 Protein Interaction on NO_3 ~ - N Uptake by Rice. The results showed that under aluminum stress, the activities of H ~ + -ATPase, H ~ + - pump, the interaction between plasma membrane H ~ + -ATPase and 14-3-3 protein decreased, the contents of H_2O_2 and MDA Content increased, leading to reduced rice NO_3 ~ - N absorption. FC treatment could increase H ~ + -ATPase hydrolytic activity, H ~ + - pump activity and the interaction of plasma membrane H ~ + -ATPase and 14-3-3 protein under aluminum stress, while the content of H 2 O 2 and MDA decreased , So that rice NO_3 ~ - N absorption increased. These results show that aluminum stress affects the plasma membrane H ~ + -ATPase hydrolytic activity, hydrogen pump activity, H 2 O 2 and MDA content by affecting the plasma membrane H ~ + -ATPase interaction with 14-3-3 protein, Nitrogen absorption.