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作者报道了一种命名为抗-D66的单克隆抗体,认为它是对位于淋巴细胞表面参与E玫瑰花形成的一抗原决定簇(D66抗原)。抗-D66单克隆抗体是由人胸腺细胞免疫小鼠后,取其脾细胞与Mopc21NS1/1瘤细胞融合,经过筛选产生的,为IgG2b亚类。预先将抗-D66同T淋巴细胞温育,会抑制在4℃和37℃时E花环形成。有趣的是,用细胞有丝分裂素刺激T细胞后,其表面D66抗原密度的增加同时伴随着37℃稳定玫瑰花形
The authors reported a monoclonal antibody, named anti-D66, which is thought to be an epitope (D66 antigen) that is involved in the formation of E-rose on the surface of lymphocytes. Anti-D66 monoclonal antibody is obtained from human thymocytes immunized mice, the spleen cells were fused with Mopc21NS1 / 1 tumor cells, after screening for IgG2b subclass. Pre-incubation of anti-D66 with T lymphocytes inhibited E-rosette formation at 4 ° C and 37 ° C. Interestingly, after stimulating T cells with mitogen, the increase in the density of D66 antigen on their surface accompanied by a steady rosette at 37 ° C