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磷酸烯醇式丙酮酸羧化酶(PEPCase)是C4双羧酸循环途径中CO2固定初始反应的一种酶,在该酶作用下,C4植物的光合速率,特别是在强光高温条件下,明显高于C3植物。甘蔗属于禾本科甘蔗属,为C4作物之一。本研究对转甘蔗pepc基因水稻恢复系N175的转基因后代进行遗传学分析,Southernblot检测结果表明,甘蔗pepc基因已经整合到水稻恢复系N175基因组中,在一些转基因株系中,甘蔗pepc基因以单拷贝的方式整合到杂交水稻恢复系N175的基因组中。对转甘蔗pepc基因的水稻恢复系N175株系进行光合效率分析,结果表明转基因株系的光合效率显著高于非转基因对照。在转基因株系中,最高的光合效率为26.50μmol·m-2·s-1,与非转基因对照相比,光合效率提高了106%,说明甘蔗的pepc基因在一定程度上可以提高转基因水稻的光合效率。
Phosphoenolpyruvate carboxylase (PEPCase) is an enzyme that catalyzes the initial CO2 fixation in the C4 dicarboxylic acid cycle. Under the action of this enzyme, the photosynthetic rate of C4 plants, especially under high light and high temperature, Significantly higher than C3 plants. Sugarcane belongs to the genus Sugarcane, one of the C4 crops. In this study, the transgenic progenies of N175 transgenic rice lines of transgenic sugarcane pepc were genetically analyzed. Southern blot analysis showed that the sugarcane pepc gene was integrated into the N175 genome of rice restorer lines. In some transgenic lines, the sugarcane pepc gene was expressed as a single copy Into the genome of hybrid rice restorer line N175. The photosynthetic efficiency of rice restorer line N175 which transfered the sugarcane pepc gene was studied. The results showed that the photosynthetic efficiency of the transgenic lines was significantly higher than that of the non-transgenic control. In the transgenic lines, the highest photosynthetic efficiency was 26.50μmol · m-2 · s-1, compared with non-transgenic control, the photosynthetic efficiency increased by 106%, indicating that the pepc sugarcane sugarcane gene to some extent can improve the transgenic rice Photosynthetic efficiency.