论文部分内容阅读
目的：分别以红藻氨酸（ＫＡ）微注射和γ－刀照射大鼠单侧尾状核，比较两者星形胶质细胞（ＡＳ）的适应性变化．方法：用抗胶质纤维酸性蛋白（ＧＦＡＰ）的ＡＢＣ法检测在受损伤３ｈ至３０ｄ的不同时期ＡＳ的变化．结果：２种损伤方式都可引起靶区ＧＦＡＰ阳性反应的细胞数量的增加，并且都可见到两种类型的阳性细胞，即胞体小、突起细的纤维型和胞体大、突起粗的肥大型．但二者在ＡＳ的反应顺序上有不同，在ＫＡ微注射组，２４ｈ内靶区呈大片坏死，几乎无阳性反应出现，３ｄ后坏死区周围有少量ＡＳ出现，随成活时间延长，ＡＳ的增生和肥大增加；而γ－刀照射组，靶区在３ｈ～７ｄ时都有ＡＳ的增生和肥大，１４ｄ时靶区有坏死点出现，ＡＳ数量比３ｈ～７ｄ时减少，３０ｄ时出现坏死区，且在坏死区周围ＡＳ反应呈层次变化．距离靶区越近，ＡＳ变形越明显，距靶区越远，ＡＳ渐呈现正常细胞形态．结论：γ射线和ＫＡ在损伤大鼠脑组织后可引起星形胶质化反应；ＧＦＡＰ可以作为一种衡量ＣＮＳ损伤的指标；两者反应的不同之处则提示二者的作用机理不同．
OBJECTIVE: To investigate the adaptive changes of both astrocytes (AS) in rats by injecting kainic acid (KA) and γ-knife into unilateral caudate nucleus. Methods: ABC of glial fibrillary acidic protein (GFAP) was used to detect the changes of AS in different time from 3h to 30d. Results: Two kinds of injury modes could all induce the increase of GFAP positive cells in the target area. Both types of positive cells could be found, that is, small cell body, fine prominence fiber type and big cell body with thick protuberances. However, there was a difference in the order of reaction between AS and KA. In the KA microinjection group, the target area was largely necrotic within 24 hours and almost no positive reaction occurred. After 3 days, a small amount of AS appeared around the necrotic area. As the survival time prolonged, AS proliferated And hypertrophy increased; and γ-knife irradiation group, the target area in the 3h ~ 7d have AS hyperplasia and hypertrophy, the target area 14d necrosis appeared, AS number decreased than 3h ~ 7d, necrosis area 30d, And in the necrotic area around the AS reaction was hierarchical changes. The closer to the target area, the more obvious the AS deformation. The farther away from the target area, AS gradually showed normal cell morphology. CONCLUSION: γ-ray and KA can cause astrogliosis in rat brain after injury; GFAP can be used as a measure of CNS damage; the difference between the two reactions suggests that the mechanism of action of both are different.