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AIM:To explore the role and significance of costimulatorymolecules B7H1,B7H2 and ICOS within tissues of humangastric carcinoma and the possible mechanisms in tumorescape.METHODS:mRNA expressions of costimulatory moleculesincluding B7H1,B7H2,ICOS and B7-1 in tissues of humangastric carcinoma were investigated by in situ hybridizationusing digoxigenin-labeled oligonucleotide-probes.The tissueof chronic gastric ulcer was used as a control.All data wereanalyzed by SPSS statistic software.RESULTS:At the site of gastric carcinoma,mRNAexpression levels of B7H1,B7H2 and ICOS were muchhigher than that of B7-1.Their mRNA positive expressionindexes were 0.512±0.333,0.812±0.454,0.702±0.359 and0.293±0.253,respectively.The positively stained cells weremainly tumor infiltrating lymphocytes (TILs),and sometumor cells.The difference between them was greatlysignificant P<0.005.The mRNA expression levels of fourmolecules were not correlated to the pathological gradeand matastasis of gastric carcinoma.CONCLUSION:ICOS-B7H costimulatory pathway may bepredominant at the site of gastric carcinoma.B7-1mRNAmight be the basis of ICOS-B7H interaction.ICOS-B7Hinteraction induces the production of IL-10 which inhibitsthe antitumor immune responses.Therefore,it is supposedthat ICOS-B7H costimulatory pathway may be involved inthe negative regulation of cell-mediated immune responses.
AIM: To explore the role and significance of costimulatory molecules B7H1, B7H2 and ICOS within tissues of humangastric carcinoma and the possible mechanisms in tumorescape. METHODS: mRNA expressions of costimulatory molecules including B7H1, B7H2, ICOS and B7-1 in tissues of humangastric carcinoma were investigated by in situ hybridization using digoxigenin-labeled oligonucleotide-probes. All tissue were dried gastric ulcer was used as a control. All data were analyzed by SPSS statistic software. RESULTS: At the site of gastric carcinoma, mRNA expression levels of B7H1, B7H2 and ICOS were muchhigher than that of B7-1.Their mRNA positive expressionindexes were 0.512 ± 0.333, 0.812 ± 0.454, 0.702 ± 0.359 and 0.293 ± 0.253, respectively. The positively stained cells were primarily tumor infiltrating lymphocytes (TILs), and sometumor cells. The difference between them was greatlysignificant P <0.005. The mRNA expression levels of four molecules were not correlated to the pathological grade and matastasis of gastric carcinoma .CONCLUSION: ICOS-B7H costimulatory pathway may be predominant at the site of gastric carcinoma. B7-1 mRNA might be the basis of ICOS-B7H interaction. ICOS-B7 Interaction induces the production of IL-10 which inhibit stage of antitumor immune responses. Herefore, it is supposedthat ICOS-B7H costimulatory pathway may be involved inthe negative regulation of cell-mediated immune responses.