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短小棒状杆菌(Corynebacteria Parvum,CP)是一种强有力免疫激活剂,本文是以其裂解产物(CPCW)采用双盲方法,在其基本治疗相同的情况下,佐以 CPCW 治疗,现报导如下:一、材料和方法(一)CPCW 的制作:从正常人骨髓中分离的菌号为 H84菌种接种于液体培养基中,37℃增菌培养7天,离心收集沉淀,经甲醛灭活后制成 CP 菌苗,后按 Azuma 方法,将1%CP 菌悬液置冰浴中,用 meE、Soninprep150超声波碎化25万赫兹下裂解30分钟,将经裂解的混悬液800克离心15分钟,上清液为细胞壁和细胞浆成份,沉淀物为未破碎细胞,将上清液20000g低温离心60分钟,沉淀物为粗制细胞壁,用 DX—
Corynebacteria Parvum (CP) is a potent immunostimulant. In this paper, a double-blind method based on its cleavage product (CPCW) and CPCW treatment under the same basic treatment is reported as follows: Materials and Methods (1) Production of CPCW: The strain No. H84 isolated from the normal human bone marrow was inoculated into a liquid medium and cultured at 37 ° C for 7 days. The precipitate was collected by centrifugation and inactivated by formaldehyde Into CP vaccine, according to Azuma method, the 1% CP suspension was placed in an ice bath, with meE, Soninprep150 250,000 hertz ultrasound fragmentation for 30 minutes, the lyophilized suspension was centrifuged at 800 g for 15 minutes, The supernatant was composed of cell wall and cytoplasm, the pellet was unbroken, the supernatant was centrifuged at 20000 g for 60 min at low temperature, the pellet was a crude cell wall and treated with DX-