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目的探讨不同浓度异丙酚对哮喘大鼠氧化应激反应及气道炎症的影响。方法清洁级SD大鼠32只随机分为正常组、模型组、低剂量组、高剂量组,每组大鼠8只。正常组给予等量生理盐水灌胃;模型组给予等量生理盐水灌胃,且于22 d雾化吸入后腹腔注射50 mg/kg戊巴比妥钠麻醉;低剂量组、高剂量组分别给予25 mg·kg~(-1)·h~(-1)和50 mg·kg~(-1)·h~(-1),静脉滴注。各组大鼠均在治疗后7 d取器官组织行HE染色观察。比较各组大鼠血清白介素(IL)-5、分离嗜酸性粒细胞(EOS)、免疫球蛋白(Ig)E、IL-4水平及肺组织匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)水平变化。结果与正常组比较,模型组、低剂量组、高剂量组IL-5、EOS、Ig E和IL-4水平明显增加,差异有统计学意义(P<0.05);低剂量组和高剂量组IL-5、EOS、Ig E和IL-4水平明显低于模型组(P<0.05);高剂量组IL-5、EOS、Ig E和IL-4水平明显低于低剂量组(P<0.05);与正常组比较,模型组、低剂量组、高剂量组MDA水平明显增加,而SOD水平明显降低(P<0.05);低剂量组和高剂量组MDA水平明显低于模型组,而SOD水平明显高于模型组(P<0.05);高剂量组MDA水平明显低于低剂量组,而SOD水平明显高于低剂量组(P<0.05)。结论不同浓度异丙酚可抑制哮喘模型大鼠增高的氧化应激反应,以及缓解大鼠气道炎症,其中以50 mg·kg~(-1)·h~(-1)浓度的异丙酚效果更为明显。
Objective To investigate the effects of different concentrations of propofol on oxidative stress and airway inflammation in asthmatic rats. Methods Thirty-two SD rats were randomly divided into normal group, model group, low dose group and high dose group, 8 rats in each group. The rats in the normal group were treated with the same volume of saline, the rats in the model group were given the same amount of saline, and were injected with 50 mg / kg pentobarbital sodium intraperitoneally after 22 days of inhalation. The rats in the low and high dose groups were given respectively 25 mg · kg -1 · h -1 and 50 mg · kg -1 · h -1 intravenously. The rats in each group were examined by HE staining 7 days after treatment. The levels of serum interleukin (IL) -5, eosinophil (EOS), immunoglobulin (E), IL-4 and lung tissue homogenate superoxide dismutase (SOD) Aldehyde (MDA), nitric oxide (NO) levels change. Results Compared with the normal group, the levels of IL-5, EOS, IgE and IL-4 in the model group, low dose group and high dose group were significantly increased (P <0.05); the low dose group and the high dose group The levels of IL-5, EOS, Ig E and IL-4 in the high dose group were significantly lower than those in the low dose group (P <0.05) ). Compared with normal group, MDA level in model group, low dose group and high dose group increased significantly and SOD level decreased significantly (P <0.05); MDA level in low dose group and high dose group was significantly lower than that in model group, while SOD (P <0.05). The level of MDA in the high dose group was significantly lower than that in the low dose group, while the level of SOD in the high dose group was significantly higher than that in the low dose group (P <0.05). Conclusions Propofol at different concentrations can inhibit the increased oxidative stress in asthmatic rats and relieve the airway inflammation in rats. Propofol at a concentration of 50 mg · kg -1 · h -1 The effect is more obvious.