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目的 探讨N hydroxyphenyl retinamide(4HPR)对人胆管癌细胞QBC939细胞增殖和凋亡的影响及其可能机制。方法 采用四甲基氮唑蓝实验 (MTT)、免疫组织化学染色、流式细胞术等技术 ,检测经 5× 10 -6mol/L 4HPR处理后 ,QBC939细胞生长增殖、细胞周期和凋亡的变化 ,以及对P2 1waf1、P2 7kip1、P5 3蛋白表达的影响。结果 4HPR显著抑制人胆管癌细胞QBC939细胞的生长增殖 ,并呈时间、剂量依赖性。用 5× 10 -6mol/L 4HPR处理细胞后 ,细胞周期停滞于G1期。流式细胞术检测发现 ,4HPR处理 12h细胞凋亡率达 9 7% ,至 4 8h达 5 7 2 %。而P2 1waf1、P2 7kip1蛋白表达呈上升趋势 ,P5 3蛋白表达则无明显变化。结论 4HPR具有抗增殖和诱导QBC939细胞发生凋亡的作用 ,并可导致细胞周期停滞于G1期。此种机制与其上调P2 1waf1、P2 7kip1蛋白表达有关 ,而与P5 3蛋白表达无关。
Objective To investigate the effect of N hydroxyphenyl retinamide (4HPR) on the proliferation and apoptosis of human cholangiocarcinoma cell line QBC939 and its possible mechanism. Methods MTT, immunohistochemistry and flow cytometry were used to detect the changes of proliferation, cell cycle and apoptosis in QBC939 cells treated with 5 × 10 -6 mol / L 4HPR , As well as on the P2 1waf1, P2 7kip1, P5 3 protein expression. Results 4HPR significantly inhibited the growth and proliferation of human cholangiocarcinoma cells QBC939 cells in a time-and dose-dependent manner. After treatment with 5 × 10 -6 mol / L 4HPR, the cell cycle arrests in the G1 phase. The results of flow cytometry showed that the apoptotic rates of 4HPR-treated cells reached 97% at 12h and reached 572% at 48h. However, the expression of P2 1waf1 and P2 7kip1 protein showed an upward trend, while P5 3 protein expression did not change significantly. Conclusion 4HPR can inhibit the proliferation of QBC939 cells and induce cell cycle arrest in G1 phase. This mechanism is related to its up-regulation of P2 1waf1 and P2 7kip1 protein expression, but not to P5 3 protein expression.