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[目的]筛选适宜加拿大披碱草×肥披碱草种间杂种F1愈伤组织诱导、幼苗分化和生根的最适培养基。[方法]以加拿大披碱草×肥披碱草种间杂种F1幼穗为外植体,以MS为基本培养基,添加不同的激素和营养物,探讨了2,4-D、6-BA、NAA与IBA4种激素不同浓度对杂种F1愈伤组织的诱导、分化及生根的影响,建立了组培再生体系。[结果]激素2,4-D对杂种F1愈伤组织诱导起决定性作用。适宜杂种F1愈伤组织诱导的最佳培养基为:MS+CH300.0 mg/L+2,4-D2.0 mg/L,诱导率达100%;适宜幼苗分化的最佳培养基为:MS+CH300.0 mg/L+6-BA2.0 mg/L+NAA0.4 mg/L,分化率达73.3%;适宜生根的最佳培养基为:MS+CH300.0 mg/L+IBA0.5 mg/L,生根率达86.7%;组培苗经过炼苗移栽得到了完整植株。[结论]杂种F1组培再生体系的建立为新种质的保存奠定了基础。2,4-D适宜浓度为2.0 mg/L。
[Objective] The research aimed to screen the optimum medium for callus induction, seedling differentiation and rooting of interspecific F1 hybrids of Elymus x Elymus. [Method] With the spikelets of F1 hybrid between Elymus x Ephylostella as the explants and MS as the basic medium, different hormones and nutrients were added to investigate the effects of 2,4-D, 6-BA , NAA and IBA hormones on the induction of callus F1 hybrids, differentiation and rooting established tissue regeneration system. [Result] The hormone 2,4-D played a decisive role in the induction of hybrid F1 callus. The optimum medium for the induction of hybrid F1 callus was as follows: MS + CH300.0 mg / L + 2,4-D2.0 mg / L, the induction rate of 100%; suitable for seedling differentiation of the best medium: MS + CH300.0 mg / L + 6-BA2.0 mg / L + NAA0.4 mg / L, the rate of differentiation was 73.3%. The best medium for rooting was MS + CH300.0 mg / L + IBA0 .5 mg / L, the rooting rate was 86.7%. The whole plantlets were obtained after the seedlings were transplanted. [Conclusion] The establishment of hybrid F1 regeneration system laid the foundation for the preservation of new germplasm. The optimum concentration of 2,4-D was 2.0 mg / L.