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目的探讨残留水分对外科器械二次污染发生率的影响。方法将手术器械分别置于可重复使用灭菌容器与双层无纺灭菌包中。并设置破坏了包装屏障完整性阳性对照与密封塑料的阴性对照,在蒸汽消毒后0,2,4,8,16天取出包装,并对包装内的陶瓷载体与残余水进行培养。结果灭菌后可重复使用的容器内的残余水平均值为28.5 m L,双层无纺灭菌包内残余水为39.1 m L。两种包装实验样品内的残留水和陶瓷载体均未培养出微生物。可重复使用的容器内的阳性对照样品在存储0天,2天和4天后发现微生物污染。阴性对照样品则始终保持无菌状态。双层无纺布灭菌包内的载体和残余水样品(包括阳性和阴性对照)培养结果均为阴性。结论灭菌后残余水分并不会增加外科器械在随后的储存期间发生二次污染的概率。
Objective To investigate the effect of residual moisture on the incidence of secondary contamination of surgical instruments. Methods The surgical instruments were placed in reusable sterile containers and double-layer non-woven sterile package. And set up to destroy the integrity of the packaging barrier positive control and sealed plastic negative control, 0,2,4,8,16 days after steam sterilization to remove the package, and the packaging of ceramic carriers and residual water culture. Results The average residual level of the reusable containers after sterilization was 28.5 m L, and the residual water in the double-layer non-woven sterilization package was 39.1 m L. Neither the residual water nor the ceramic carrier in the two packaging experimental samples cultured the microorganisms. Positive control samples in reusable containers found microbial contamination after 0 days, 2 days and 4 days of storage. Negative control samples are always sterile. Both the carrier and residual water samples (including positive and negative controls) in the bi-layer non-woven sterilization package were negative in culture. Conclusion The residual moisture after sterilization does not increase the probability of secondary contamination of surgical instruments during subsequent storage.